{"id":353,"date":"2023-11-15T16:28:26","date_gmt":"2023-11-15T07:28:26","guid":{"rendered":"https:\/\/www.cib.bio.titech.ac.jp\/?page_id=353"},"modified":"2025-04-28T12:02:56","modified_gmt":"2025-04-28T03:02:56","slug":"synbio-foundry","status":"publish","type":"page","link":"https:\/\/www.cib.bio.titech.ac.jp\/en\/facilities\/synbio-foundry\/","title":{"rendered":"Cell Analysis Room"},"content":{"rendered":"\n

Cell Sorter<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Becton Dickinson<\/td><\/tr>
Model number<\/th>FACS Melody<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Each cell is irradiated by laser and analyzed using fluorescence and scattered light.
The results are then used to sort the target cells.<\/td><\/tr>
Spec<\/th>lasers\uff1a405 nm, 488 nm, 561 nm
Maximum analysis speed\uff1a40,000 events\/sec (catalog value)
Nozzle diameter\uff1a100 \u03bcm
Capable of simultaneously sorting two types of tubes and single cell sorting on 96 well plate.<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th>New users are required to attend a training session.
In case of significant repair costs, the amount will be added based on the usage time.<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Analysis Software Flow Jo<\/h2>\n\n\n\n
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Manufacturer<\/th>Becton Dickinson<\/td><\/tr>
Model number<\/th>Flow Jo<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Analyzes any fcs file from any cytometer.<\/td><\/tr>
Spec<\/th>Example data analysis\uff1aImmunophenotyping, Cell cycle, Proliferation, Kinetics studies, Quantitative population comparison, Plate screening assay<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Automatic Cell Culture Observation System<\/h2>\n\n\n\n
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\n
Manufacturer<\/th>Nikon<\/td><\/tr>
Model number<\/th>BioStation CT<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Cells can be observed and images acquired while incubated in a stable environment in an incubator.<\/td><\/tr>
Spec<\/th>Observation: Phase contrast, Fluorescence
Image acquisition: Capable of acquiring images of the entire container area.
Containers: Multi well plate
Fluorescence filter: GFP, DsRed, YFP, TexasRed<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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384-well Realtime PCR<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Thermo Fisher Scientific<\/td><\/tr>
Model number<\/th>QuantStudio6Pro<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Quantify DNA and RNA by monitoring and measuring PCR progress in real time.
Very small changes in gene expression can be detected.<\/td><\/tr>
Spec<\/th>Reaction volume (recommended): 384 well plate 10\uff5e20 \u03bcl\u300196 well plate 20 \u03bcl
Equipped with 5 fluorescent filters.<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Automated Pipetting System<\/h2>\n\n\n\n
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Manufacturer<\/th>Analytikjena<\/td><\/tr>
Model number<\/th>CyBio Felix Choice<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Flexible pipetting platform with fully automated single to multi-channel liquid handling
Ideal for PCR setup and NGS prep etc.<\/td><\/tr>
Spec<\/th>Containers: 96\u3001384 well plate\u30010.2\u30010.5\u30011 ml tube
Dispensing range: 1\uff5e1000 \u03bcl<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Electrophoresis Equipment<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Agilent Technologies Japan<\/td><\/tr>
Model number<\/th>4200TapeStation<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Automated electrophoresis system for quality control (QC) of DNA and RNA samples.
Requires less than 2 \u00b5L of sample, saving valuable specimens<\/td><\/tr>
Spec<\/th>Containers: 8 row tube, 96 well plate
Measuring volume: 1\uff5e2 \u03bcl
Measuring time: 96 samples in 90 minutes
Analysis range of each kit: [Genomic DNA] 200-60,000 bp, [Cell free DNA] 50-800 bp, [RNA] 100-6,000 bp, [D5000] 100-5,000 bp, [D1000] 35-1,000 bp<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Cryostat CM3050S<\/h2>\n\n\n\n
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\n
Manufacturer<\/th>Leica Biosystems<\/td><\/tr>
Model number<\/th>CM3050S<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Thinly slice Frozen specimen and prepare sections for Immunohistochemistry, etc.<\/td><\/tr>
Spec<\/th>Temperature range: -10 \uff5e -50 \u2103
Section thickness setting: 0.5\uff5e300 \u03bcm
Trimming thickness setting: 5\uff5e150 \u03bcm
Overall specimen feed: 25 mm
Vertical stroke: 29 mm
Maximum specimen size: 40\u00d755 mm
Motorized coarse feed: slow 500 \u03bcm\/sec, fast 1,000 \u03bcm\/sec
Function: Retraction<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking <\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th>Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Bio Clean Bench<\/h2>\n\n\n\n
\n
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Manufacturer<\/th>Panasonic Healthcare (PHC)<\/td><\/tr>
Model number<\/th>MCV\uff70B161F-PJ<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Air flows through HEPA filter into the chamber to maintain sterile conditions.
P1 level experiments are possible.<\/td><\/tr>
Spec<\/th>Airflow: Vertical laminar flow<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Class\u2161 Safety Cabinet<\/h2>\n\n\n\n
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Manufacturer<\/th>Thermo Fisher Scientific<\/td><\/tr>
Model number<\/th>1300 Series Class II, Type A2 biological safety cabinet SS 1375<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Protects researcher, sample, and environment from contamination by hazardous substances and allows for aseptic manipulation.<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Microvolume Spectrophotometer<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Thermo Fisher Scientific K.K.<\/td><\/tr>
Model number<\/th>NanoDrop Eight<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Spectroscopic measurement of the concentration of biological samples such as nucleic acids and proteins etc.
8 samples at a time in less than 20 seconds.<\/td><\/tr>
Spec<\/th>Light source: LED
Measuring wavelength: 220-750 nm
Measuring volume: 1 \u03bcl minimum (1.5 \uff5e2 \u03bcl recommended)
Up to 8 samples can be measured at one time<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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Automated Cell Counter<\/h2>\n\n\n\n
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\n
Manufacturer<\/th>Scrum<\/td><\/tr>
Model number<\/th>Cell Drop FL<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Automated cell counter based on micro-spectrophotometer technology.
No disposable slides are used, and the cell suspension can be placed directly on the measurement site.<\/td><\/tr>
Spec<\/th>Measurement time: About 3\uff5e8.5seconds
Measurement concentration: 7\u00d7102\uff5e2.5\u00d7107 cells\/mL<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th>https:\/\/chem-eqnet.ims.ac.jp\/<\/a><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
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CO2 Incubator<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Panasonic Healthcare (PHC)<\/td><\/tr>
Model number<\/th>MCO-170 AICUV-PJ<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>CO2<\/sub> incubator for cell culture<\/td><\/tr>
Spec<\/th>Heating method: Heater jacket+air jacket method
Humidification method: Humidification tray natural evaporation method
Temperature: Ambient temperature +5 \u2103~50 \u2103
CO2<\/sub> control range: 0 %-20 %
Capacity: 165 L<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
\n
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Refrigerator\/ Freezer\uff08Bio-Multi Cooler\uff09<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Nihon Freezer<\/td><\/tr>
Model number<\/th>KGT-370HC<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Used for storing cell culture-related reagents, etc.<\/td><\/tr>
Spec<\/th>(Refrigerator)
Temperature: 3 \uff5e 16 \u2103
Capacity: 267 L
(Freezer)
Temperature: -9 \u2103 \uff5e -30 \u2103
Capacity: 110 L<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
\n
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\n

<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n

-80\u2103\u3000Freezer<\/h2>\n\n\n\n
\n
\n
Manufacturer<\/th>Nihon Freezer<\/td><\/tr>
Model number<\/th>CLN-52UD1<\/td><\/tr>
Type of use<\/th>self-use<\/td><\/tr>
Overview<\/th>Used for long-term storage of biological samples such as DNA, proteins, and cell extracts.<\/td><\/tr>
Spec<\/th>Temperature: -70 \u2103 \uff5e -80 \u2103
Capacity: 552 L<\/td><\/tr>
Outside User<\/th>Impossible<\/td><\/tr>
Booking<\/th><\/td><\/tr>
Remarks<\/th><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
\n
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<\/p>\n","protected":false},"excerpt":{"rendered":"

Cell Sorter Analysis Software Flow Jo Automatic Cell Culture Observation System 384-well Realtime PCR Automated Pipetting System Electrophoresis Equipment Cryostat CM3050S Bio Clean Bench Class\u2161 Safety Cabinet Microvolume Spectrophotometer Automated Cell Counter CO2 Incubator Refrigerator\/ Freezer\uff08Bio-Multi Cooler\uff09 -80\u2103\u3000Freezer<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":325,"menu_order":19,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"_locale":"en_US","_original_post":"https:\/\/www.cib.bio.titech.ac.jp\/?page_id=79","footnotes":""},"class_list":["post-353","page","type-page","status-publish","hentry","en-US"],"yoast_head":"\nCell Analysis Room | \u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u7d71\u5408\u652f\u63f4\u65bd\u8a2d | \u6771\u4eac\u79d1\u5b66\u5927\u5b66<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, 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