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Bioimaging Room

Inverted Laser Scanning Confocal Microscope

Manufacturer	Carl Zeiss Microscopy
Model number	LSM780 with Airyscan
Type of use	self-use
Overview	Inverted microscope AxioObserverZ1 system with confocal laser scanning microscope LSM780 and Multi-functional ultra-sensitive array GaAsP detector Airyscan
Spec	Objective: Plan-Apochromat 10x/0.45, 20x/0.8, 40x/1.4Oil, 63x/1.4Oil, 100x/1.46Oil, C-Apochromat 40x/1.2W Laser: Diode 405nm (25mW), Ar 458/488/514nm (25mW), DPSS 561nm (20mW), HeNe 633nm (5mW) Detector: 32ch (GaAsP) + 2ch for fluorescence, 1ch for transmitted light, Airyscan Others: motorised stage, stage top incubator
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Upright Laser Scanning Confocal Microscope

Manufacturer	Carl Zeiss Microscopy
Model number	LSM780
Type of use	self-use
Overview	Upright microscope AxioImagerZ2 system with confocal laser scanning microscope LSM780
Spec	Objective: Plan-Apochromat 10x/0.45, 20x/0.8, 40x/1.4Oil, 63x/1.4Oil, W-Plan-Apochromat 40x/1.0 Laser: Diode 405nm (25mW), Ar 458/488/514nm (25mW), DPSS 561nm (20mW), HeNe 633nm (5mW) Detector: 32ch (GaAsP) + 2ch for fluorescence, 1ch for transmitted light
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

High Resolution Inverted Fluorescence Microscope

Manufacturer	Carl Zeiss Microscopy
Model number	Axio Observer Z1
Type of use	self-use
Overview	Inverted microscope AxioObserverZ1 system with high-sensitivity CCD camera
Spec	Objective: EC Plan-Neofluar 10xPh/0.3, LD20xPh/0.4, LD40xPh/0.6, Plan-Apochromat 63x/1.4Oil Filter: DAPI, GFP, RFP, Cy5. Light source: LED 365nm, 470nm, 540-580nm, 625nm Camera: high-sensitivity CCD camera ORCA Flash4 Function: superimposition, time-lapse, deconvolution
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Zoom Microscope

Manufacturer	Carl Zeiss Microscopy
Model number	Axio Zoom V16
Type of use	self-use
Overview	Zoom microscope system with high-sensitivity CCD camera
Spec	Objective : 1.0x/0.25FWD56mm, 2.3x/0.57FWD10.6mm. Fluorescence filters: DAPI, GFP, DsRed Camera: high-sensitivity CCD camera AxioCamMRm Function: superimposition, tiling, Z-stack
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Microscopy Image Analysis Software IMARIS1

Manufacturer	Carl Zeiss Microscopy
Model number	IMARIS
Type of use	self-use
Overview	Extensive display and analysis of 3D/4D images created by confocal laser scanning microscopes
Spec	Imaris
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Microscopy Image Analysis Software IMARIS2

Manufacturer	Carl Zeiss Microscopy
Model number	IMARIS2
Type of use	self-use
Overview	Extensive display and analysis of 3D/4D images created by confocal laser scanning microscopes
Spec	Imaris, Measurement Pro, Coloc, Filament Tracer, Cell
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Rotary Microtome

Manufacturer	YAMATO KOHKI
Model number	RX-860
Type of use	self-use
Overview	Rotary microtome developed for thin-slicing performance
Spec	Slice thickness setting range: 0.1 – 500.0 µm (dial system). Vertical stroke length: 70 mm Front-back stroke length: 40 mm Function: retraction, counter, trip meter, feed lock
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

3D Holotomography Microscope

Manufacturer	Tomocube
Model number	HT-2
Type of use	self-use
Overview	Label-free quantitative imaging
Spec	Lens Type: 60 X NA 1.2 (WATER IMMERSION) Lateral Resolution: 110nm Axial Resolution: 356nm Time Res.: 150 fps (2D), 2.5 fps (3D) Basic（diode laser）: 532 nm Fluorescence（LED）: Blue: 385 nm, Green: 470 nm, Red 570 nm
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Digital Imaging System

Manufacturer	Evident
Model number	APX100
Type of use	self-use
Overview	Benchtop microscope system for brightfield, phase contrast, and fluorescence observation.　APX100 supports a wide range of research imaging applications including slides, dishes, and well plates. A wide range of imaging methods, including multicolor imaging, Image Stitching, time-lapse, and Z-stacking, can be used in any combination that fits your research protocol.
Spec	Objective: 4x/0.13, 10x/0.3, 20x/0.7 WD0.8-1.8mm, Apocromat 40x/0.95, Apocromat 100x/1.45 Oil Transillumination: Köehler illumination, LED light source Stage: Motorized XY stage Filters: B excitation (U-FBNA), U excitation (U-FUNA), G excitation (U-FGW), CY5 (U-DM3-CY5) Camera: 1/1.8″ 6.41 megapixel color CMOS, 1/1.8″ 6.41 megapixel monochrome CMOS
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.