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Cell Analysis Room

Cell Sorter

Manufacturer	Becton Dickinson
Model number	FACS Melody
Type of use	self-use
Overview	Each cell is irradiated by laser and analyzed using fluorescence and scattered light. The results are then used to sort the target cells.
Spec	lasers：405 nm, 488 nm, 561 nm Maximum analysis speed：40,000 events/sec (catalog value) Nozzle diameter：100 μm Capable of simultaneously sorting two types of tubes and single cell sorting on 96 well plate.
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	New users are required to attend a training session. In case of significant repair costs, the amount will be added based on the usage time.

Analysis Software Flow Jo

Manufacturer	Becton Dickinson
Model number	Flow Jo
Type of use	self-use
Overview	Analyzes any fcs file from any cytometer.
Spec	Example data analysis：Immunophenotyping, Cell cycle, Proliferation, Kinetics studies, Quantitative population comparison, Plate screening assay
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

Automatic Cell Culture Observation System

Manufacturer	Nikon
Model number	BioStation CT
Type of use	self-use
Overview	Cells can be observed and images acquired while incubated in a stable environment in an incubator.
Spec	Observation: Phase contrast, Fluorescence Image acquisition: Capable of acquiring images of the entire container area. Containers: Multi well plate Fluorescence filter: GFP, DsRed, YFP, TexasRed
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

384-well Realtime PCR

Manufacturer	Thermo Fisher Scientific
Model number	QuantStudio6Pro
Type of use	self-use
Overview	Quantify DNA and RNA by monitoring and measuring PCR progress in real time. Very small changes in gene expression can be detected.
Spec	Reaction volume (recommended): 384 well plate 10～20 μl、96 well plate 20 μl Equipped with 5 fluorescent filters.
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

Automated Pipetting System

Manufacturer	Analytikjena
Model number	CyBio Felix Choice
Type of use	self-use
Overview	Flexible pipetting platform with fully automated single to multi-channel liquid handling Ideal for PCR setup and NGS prep etc.
Spec	Containers: 96、384 well plate、0.2、0.5、1 ml tube Dispensing range: 1～1000 μl
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

Electrophoresis Equipment

Manufacturer	Agilent Technologies Japan
Model number	4200TapeStation
Type of use	self-use
Overview	Automated electrophoresis system for quality control (QC) of DNA and RNA samples. Requires less than 2 µL of sample, saving valuable specimens
Spec	Containers: 8 row tube, 96 well plate Measuring volume: 1～2 μl Measuring time: 96 samples in 90 minutes Analysis range of each kit: [Genomic DNA] 200-60,000 bp, [Cell free DNA] 50-800 bp, [RNA] 100-6,000 bp, [D5000] 100-5,000 bp, [D1000] 35-1,000 bp
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

Cryostat CM3050S

Manufacturer	Leica Biosystems
Model number	CM3050S
Type of use	self-use
Overview	Thinly slice Frozen specimen and prepare sections for Immunohistochemistry, etc.
Spec	Temperature range: -10 ～ -50 ℃ Section thickness setting: 0.5～300 μm Trimming thickness setting: 5～150 μm Overall specimen feed: 25 mm Vertical stroke: 29 mm Maximum specimen size: 40×55 mm Motorized coarse feed: slow 500 μm/sec, fast 1,000 μm/sec Function: Retraction
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks	Training participants are required within the research laboratory. New users can use the facilities after receiving training from the training participants.

Bio Clean Bench

Manufacturer	Panasonic Healthcare (PHC)
Model number	MCVｰB161F-PJ
Type of use	self-use
Overview	Air flows through HEPA filter into the chamber to maintain sterile conditions. P1 level experiments are possible.
Spec	Airflow: Vertical laminar flow
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

ClassⅡ Safety Cabinet

Manufacturer	Thermo Fisher Scientific
Model number	1300 Series Class II, Type A2 biological safety cabinet SS 1375
Type of use	self-use
Overview	Protects researcher, sample, and environment from contamination by hazardous substances and allows for aseptic manipulation.
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

Microvolume Spectrophotometer

Manufacturer	Thermo Fisher Scientific K.K.
Model number	NanoDrop Eight
Type of use	self-use
Overview	Spectroscopic measurement of the concentration of biological samples such as nucleic acids and proteins etc. 8 samples at a time in less than 20 seconds.
Spec	Light source: LED Measuring wavelength: 220-750 nm Measuring volume: 1 μl minimum (1.5 ～2 μl recommended) Up to 8 samples can be measured at one time
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

Automated Cell Counter

Manufacturer	Scrum
Model number	Cell Drop FL
Type of use	self-use
Overview	Automated cell counter based on micro-spectrophotometer technology. No disposable slides are used, and the cell suspension can be placed directly on the measurement site.
Spec	Measurement time: About 3～8.5seconds Measurement concentration: 7×102～2.5×107 cells/mL
Outside User	Impossible
Booking	https://chem-eqnet.ims.ac.jp/
Remarks

CO2 Incubator

Manufacturer	Panasonic Healthcare (PHC)
Model number	MCO-170 AICUV-PJ
Type of use	self-use
Overview	CO₂ incubator for cell culture
Spec	Heating method: Heater jacket+air jacket method Humidification method: Humidification tray natural evaporation method Temperature: Ambient temperature +5 ℃~50 ℃ CO₂ control range: 0 %-20 % Capacity: 165 L
Outside User	Impossible
Booking
Remarks

-80℃　Freezer

Manufacturer	Nihon Freezer
Model number	CLN-52UD1
Type of use	self-use
Overview	Used for long-term storage of biological samples such as DNA, proteins, and cell extracts.
Spec	Temperature: -70 ℃ ～ -80 ℃ Capacity: 552 L
Outside User	Impossible
Booking
Remarks